Reaction of 5.49% w/v aqueous solution at 25°C. pH : 7.2±0.2
M1327: Cultural characteristics observed on addition of FD125I and FD141 after an incubation at 35-37°C for 24-48 hours.
Suspend 54.92 grams(equivalent weight of dehydrated medium per litre) in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (FD125I) and 2 vials of Fraser Supplement (FD141) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Fraser Broth Base with added supplement is recommended, as a primary as well as secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and Human feeds.